ブックタイトル教育医学 J.Educ.Health Sci.　第63巻　第3号　通巻 第289号

概要

教育医学 J.Educ.Health Sci.　第63巻　第3号　通巻 第289号

Change in Oral Antimicrobial Peptides and Neuroendocrine Response by Intense Exercisethe period of observation were small and did notdiffer between the two sessions, as indicated by amain effect of session in hemoglobin (P = 0.179,and in hematocrit (P = 0.279).2. Saliva antimicrobial profileTable 2 shows changes over time in the amountof saliva antimicrobial components expressedby four variables; direct concentration, secretionratio, osmolality ratio, and protein ratio. Noneof the baseline values (t = 0) of these measuredvariables differed significantly between sessions.Following the 60-min exercise (t = 60 to t = 180),the mean levels of HBD-2 significantly increasedin the exercise session compared with those in theresting session, as indicated by main effects oftime (all four variables; P<0.001) and of session(concentration, secretion ratio; P<0.001, osmolalityratio; P = 0.007, and protein ratio; P = 0.014),and by a time×session interaction effect (all fourvariables; P<0.001). Similarly, significant rises insaliva LL-37 were also observed (main effect oftime; P<0.001, main effect of session; P<0.001,and a time×session interaction effect; P<0.001).In contrast, saliva IgA levels did not differ fromthose in the resting session during exercise, butdecreased for 2 hrs following the exercise, asshown by main effects of time (concentration; P =0.007, secretion ratio; P = 0.022, and protein ratio;P = 0.019) and of session (concentration; P =0.015, secretion ratio; P = 0.026, osmolality ratio;P = 0.037, and protein ratio; P = 0.002), and by atime×session interaction effect (concentration; P= 0.003, secretion ratio; P=0.011, osmolality ratio;P = 0.009, and protein ratio; P = 0.037).3. Stress-related hormones assessed by salivacortisol and plasma norepinephrineSaliva cortisol and plasma norepinephrinelevels during the period of observation are shownin Table 3. In parallel with the antimicrobialpeptides, significant increases in the salivarycortisol levels were observed during the exercisesession compared with those during the restingTABLE 1 Time course of saliva flow rate, osmolality, protein, and blood hemoglobin, hematcrit during restingand exercise sessions.t (min)0 60 120 180SalivaFlow rateRest1.54±0.191.48±0.221.51±0.251.46±0.23(mL min -1 )Exercise1.64±0.321.48±0.191.58±0.181.61±0.16OsmolalityRest76.4±8.777.9±6.078.4±9.777.1±13.6(mOsmol kg -1 )Exercise83.6±5.590.8±17.292.5±14.691.3±13.1Total proteinRest0.80±0.160.80±0.160.79±0.130.85±0.16(mg mL -1 )**Exercise0.95±0.161.38±0.12††1.05±0.201.04±0.23BloodHemoglobinRest15.2±0.715.3±0.615.6±0.715.5±0.5(g dL -1 )Exercise15.7±1.115.7±1.215.6±0.915.8±0.9HematocritRest44.5±1.345.2±1.544.8±1.344.6±1.3(%)Exercise46.1±1.846.8±1.445.5±1.145.5±1.2All values are described as mean±SD. Analysis was performed by two-way ANOVA for repeated measures. Significant main effects of time (P<0.001) and of session(P<0.001) and the time×session interaction effect (P<0.001) were detected on saliva protein levels (**P<0.01; resting vs. exercise session,††P<0.01; vs. t=0 by post-hoctest). Although there was a tendency of saliva flow rate to decrease and that of saliva osmolality to increase immediately after the exercise session (t=60), no significanteffects of time and session were observed on these two parameters. Similarly, there were no significant effects of time and session on blood hemoglobin and hematcrit.? 232 ?