ブックタイトル教育医学 J.Educ.Health Sci.　第63巻　第3号　通巻 第289号

概要

教育医学 J.Educ.Health Sci.　第63巻　第3号　通巻 第289号

Tatsuya USUI, J. Educ. Shintaro Health TSUJI, Sci., 63（3），227?240 Nobuhito NAGAI（February，2018）and Chie TAKEYASU原著Change in Oral Antimicrobial Peptides and NeuroendocrineResponse by Intense ExerciseTatsuya USUI 1) , Shintaro TSUJI 1) , Nobuhito NAGAI 1) and Chie TAKEYASU 2)AbstractThe aim of the present study was to examine whether the amount of oral antimicrobial components,humanβ-defensin-2 (HBD-2), LL-37, and immunoglobulin A (IgA) might be affected by a single boutof intense exercise, and to determine whether the activities of two main neuroendocrinological systems,the hypothalamus-pituitary-adrenal (HPA) axis and sympathetic and adrenomedullary (SA) systems, tophysical stress might be differentially or synergistically associated with these immunological changes.Seven young male volunteers either exercised on a recumbent ergometer at 75%・VO2max for 60 min(exercise session) or sat quietly (resting session). Saliva and blood samples were obtained at 60 minintervals during sessions for measurements of saliva antimicrobial components (HBD-2, LL-37, and IgA),hematocrit, and hemoglobin. Saliva cortisol and plasma norepinephrine were also determined at the sametime points as a measure of activities of the HPA axis and SA system, respectively. Saliva levels of HBD-2and LL-37 were increased during and after exercise, whereas saliva IgA was decreased after exercise.Saliva cortisol and plasma norepinephrine levels were increased during exercise. While the areas underthe curve (AUC) of the time courses of saliva levels of HBD-2 and LL-37 were positively associatedwith those of plasma norepinephrine, the AUCs of saliva HBD-2 and LL-37 were negatively associatedwith those of cortisol levels in saliva. In contrast, the decreases in salivary IgA levels were not associatedwith changes in any stress-related parameters examined. The present findings raise the possibility that theactivities of the HPA axis and the SA system during strenuous physical stress might differentially affectboth oral HBD-2 and LL-37 levels, but not those of IgA.Keywords：Antimicrobial Peptides, HBD-2, LL-37, Cortisol, Physical StressⅠ．IntroductionStrenuous endurance exercise is generallyknown to decrease the resistance to upperrespiratory tract infection (URI) 27) . The cause ofthis observation has usually been attributed tosignificant changes in several immunologicalparameters in various biological samples caused bya single bout or repeated exercise (see an excellentreview 18) ). In particular, changes in saliva levelsof immunoglobulin A (IgA) by various types ofexercise have been extensively investigated 5) .In addition to IgA, the innate immune systemconsists of various cellular and humoral factors?）大阪成蹊短期大学〒533-0007大阪市東淀川区相川3丁目10-62?）芦屋大学〒659-8511兵庫県芦屋市六麓荘町13-22?）Osaka Seikei College3-10-62, Aikawa, Higashiyodogawa, Osaka, Japan (533-0007)?）Ashiya University13-22, Rokurokusou, Ashiya-city, Hyogo, Japan (659-8511)? 227 ?